Abstract
As sessile organisms, plants are exposed to a variety of environmental stresses caused by biotic and abiotic factors during their life cycle; as a result, plants have evolved complex defense mechanisms to cope with these stresses, among which the mitogen-activated protein kinase cascade signaling pathway is particularly critical. This study focused on MdMAPK6, a specific mitogen-activated protein kinase gene in Malus domestica, to illuminate its functions in stress responses. MdMAPK6 was successfully cloned from apple and shown to respond to various stressors, including drought, salt, and abscisic acid. Overexpressing MdMAPK6 in apple calli resulted in enhanced resistance to drought, salt, and Botryosphaeria dothidea. Ectopic expression of MdMAPK6 in Arabidopsis thaliana enhanced the resistance to drought, salt, and Pseudomonas syringae pathovar tomato DC3000. These results indicated that MdMAPK6 in apples is a traditional mitogen-activated protein kinase, which plays an important role in both biotic and abiotic stresses.