A Safe and Multifunctional γ-PGA Hydrogel Platform: Endotoxin-Controlled Injectable Fillers and Antimicrobial Wound Dressings

一种安全且多功能的γ-PGA水凝胶平台:内毒素可控注射填充剂和抗菌伤口敷料

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Abstract

In response to the limitations of hyaluronic acid (HA)-such as its high cost, short durability, and instability-in anti-aging aesthetic applications, this study developed a novel injectable micelle system, with a triple network structure. It is the particle size of approximately 400 nm and the elevated potential that enhance the crosslinking density and mechanical strength of the hydrogel. Importantly, following ultrafiltration and purification processes, the material's hemolysis rate measured by spectrophotometry was only 3.25%, and endotoxin levels measured by the LAL assay were less than 0.5 EU/mL (test conditions: 37 °C, pH = 7, detection limit: 0.125 EU/mL). Building on this safe and stable material platform, we further designed an antibacterial wound dressing by functionalizing γ-PGA with penicillin or benzalkonium chloride. It reduced the cellular activity of Staphylococcus aureus by 78.9% and 84.2%, respectively. The outstanding safety profile, combined with customizable functionality, positions this γ-PGA-based platform as a promising multifunctional biomaterial meeting practical standards for both aesthetic medicine and wound care applications.

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