Abstract
Citri grandis Exocarpium (Chinese name Huajuhong, HJH) is a traditional Chinese medicinal herb widely used in traditional medicines and foods in China due to its efficacy in treating coughs and excessive phlegm. This study employed HS-SPME-GC-MS to analyze the volatile compounds in HJH samples from different regions, with the aim of distinguishing samples from Huazhou from those of other origins and exploring their potential relationship with ecological factors. A multidimensional strategy was utilized to analyze the relationships between volatile oils, climatic factors, and soil elements, examining how volatile compounds responded to ecological factors. From 47 batches of HJH samples across various regions, eight significantly different volatile compounds were identified, serving as chemical markers for HJH from Huazhou. The findings elucidate the impact of ecological factors on the volatile compounds of HJH, highlighting environmental factors relating to the authenticity of HJH from Huazhou. The results indicate that the authenticity of HJH is shaped by the unique climatic and soil environments of Huazhou.