Expression of the transmembrane mucins, MUC1, MUC4 and MUC16, in normal endometrium and in endometriosis

跨膜粘蛋白 MUC1、MUC4 和 MUC16 在正常子宫内膜和子宫内膜异位症中的表达

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作者:N Dharmaraj, P J Chapela, M Morgado, S M Hawkins, B A Lessey, S L Young, D D Carson

Methods

Total RNA was isolated from endometrial biopsies of ectopic and eutopic endometrium from women with endometriosis and control patients from different stages of the menstrual cycle. Quantitative (q)-RT-PCR analyses were performed for the mucins, MUC1, MUC4 and MUC16, relative to the epithelial marker, cytokeratin-18 (KRT18), or β-actin (ACTB). Frozen sections from endometrial biopsies of proliferative and mid-secretory stage women with endometriosis were immunostained for MUC1, MUC4 and MUC16. Main

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