Abstract
Paraisaria gracilioides (Kobayasi) Luangsa-ard, Mongkolsamrit & Samson, originally described as Ophiocordyceps gracilioides, is a member of the Ophiocordycipitaceae, within the order Hypocreales. This study, presented the mitochondrial genome of P. gracilioides structured as a circular molecule spanning 181,140 bp, was larger than those of most Ophiocordycipitaceae species. Despite this size variation, encoding 49 genes, including 15 PCGs, 26 tRNAs, two rRNAs, and six ORFs. Phylogenetic analysis based on nucleotide sequences of 14 PCGs revealed that P. gracilioides clustered with Paraisaria gracilis, forming a subgroup within Ophiocordyceps species. The results contribute to refining the taxonomic framework of Ophiocordycipitaceae.