The Cystic Fibrosis Transmembrane Conductance Regulator Gene (CFTR) Is under Post-Transcriptional Control of microRNAs: Analysis of the Effects of agomiRNAs Mimicking miR-145-5p, miR-101-3p, and miR-335-5p

囊性纤维化跨膜传导调节基因 (CFTR) 受 microRNA 的转录后控制：模拟 miR-145-5p、miR-101-3p 和 miR-335-5p 的 agomiRNA 的影响分析

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作者：Chiara Papi, Jessica Gasparello, Matteo Zurlo, Lucia Carmela Cosenza, Roberto Gambari, Alessia Finotti

期刊：

Non-Coding RNA

影响因子：

3.600

时间：

2023

起止号：

2023 Apr 18;9(2):29.

doi：

10.3390/ncrna9020029

方法学：

FCM、IF、IHC-P、WB

靶点：

VINC

研究方向：

信号转导

Background

MicroRNAs are involved in the expression of the gene encoding the chloride channel CFTR (Cystic Fibrosis Transmembrane Conductance Regulator); the

Conclusions

The treatment of target cells with the agomiR pre-miR-145-5p should be considered when CFTR gene expression should be inhibited in pathological conditions, such as polycystic kidney disease (PKD), some types of cancer, cholera, and SARS-CoV-2 infection.

Methods

CFTR mRNA was quantified by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR). The production of the CFTR protein was assessed by Western blotting; (3)

Results

The treatment of Calu-3 cells with agomiR-145-5p caused the highest inhibition of CFTR mRNA accumulation and CFTR production; (4) Conclusions: The treatment of target cells with the agomiR pre-miR-145-5p should be considered when CFTR gene expression should be inhibited in pathological conditions, such as polycystic kidney disease (PKD), some types of cancer, cholera, and SARS-CoV-2 infection.