Background
The
Conclusions
The method validated in this paper is precise, simple, and fast and can be easily adapted to automated analyzers.
Results
Imprecision was low in both apparatus utilized, and the results were linear across serial Trolox and canine serum samples dilutions. Lipids did not interfere with the assay; however, hemolysis increased the TACc concentrations. When TACc concentrations were determined in ten healthy (control) dogs and in twelve dogs with inflammatory bowel disease (IBD), dogs with IBD had lower TACc concentrations when compared with the healthy dogs. Conclusions: The method validated in this paper is precise, simple, and fast and can be easily adapted to automated analyzers.