Abstract
The tumor immune microenvironment (TIME) is closely involved in tumor initiation, malignant progression, immune escape, and response to immunotherapy. With the continued development of high-throughput sequencing technologies, transcriptomic approaches have become essential for examining the cellular and molecular features of the TIME. Bulk RNA sequencing offers tissue-level gene expression profiles and allows the estimation of immune cell composition through computational deconvolution. Single-cell RNA sequencing provides finer resolution, revealing cellular heterogeneity, lineage relationships, and functional states. Spatial transcriptomics (ST) retains the native anatomical context, making it possible to localize gene expression patterns and cell-cell interactions within intact tissues. These approaches, when considered together, have shifted TIME research from averaged measurements toward a more detailed and mechanistic understanding. This review summarizes the principles, applications and limitations of bulk, single-cell and spatial transcriptomic methods, highlighting emerging strategies for integrative analysis. Such multi-scale frameworks are increasingly important for studying immune dynamics and may contribute to the development of more precise biotechnological and immunotherapeutic strategies.