Clustering Initiated Multiphase Active Contours and Robust Separation of Nuclei Groups for Tissue Segmentation

聚类引发的多相活性轮廓和稳健的细胞核群分离用于组织分割

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Abstract

Computer assisted or automated histological grading of tissue biopsies for clinical cancer care is a long-studied but challenging problem. It requires sophisticated algorithms for image segmentation, tissue architecture characterization, global texture feature extraction, and high-dimensional clustering and classification algorithms. Currently there are no automatic image-based grading systems for quantitative pathology of cancer tissues. We describe a novel approach for tissue segmentation using fuzzy spatial clustering, vector-based multiphase level set active contours and nuclei detection using an iterative kernel voting scheme that is robust even in the case of clumped touching nuclei. Early results show that we can reach a 91% detection rate compared to manual ground truth of cell nuclei centers across a range of prostate cancer grades.

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