Abstract
PURPOSE: We investigate terahertz (THz) polarimetry imaging of seven human breast cancer surgical specimens. The goal is to enhance image contrast between adjacent tissue types of cancer, healthy collagen, and fat in excised breast tumors. Based on the biological perception of random growth of cancer and invasion of surrounding healthy tissues in the breast, we hypothesize that cancerous cells interact with the THz electric field in a different manner compared with healthy cells. This difference can be best captured using multiple polarizations instead of single polarization. APPROACH: Time domain pulsed signals are experimentally collected from each pixel of the specimen in horizontal-horizontal, vertical-horizontal, vertical-vertical, and horizontal-vertical polarizations. The time domain pulses are transformed to the frequency domain to obtain the power spectra and 16 Mueller matrix images. The whole-slide pathology imaging was used to interpret and label all images. RESULTS: The results of the cross and co-polarization power spectrum images demonstrated a strong dependency on the tissue orientation with respect to the emitted and detected electric fields. At the 130-deg rotation angle of the scanned samples, the detector showed the strongest reflected signal in cross-polarization. Furthermore, the Mueller matrix images consistently demonstrated patterns in fresh and block tissues confirming the differentiation between tissue types in breast tumor specimens. CONCLUSIONS: THz polarimetry imaging shows a potential for improving image contrast in excised tumor tissues compared with single polarization imaging. Cross-polarization signals demonstrated smaller amplitudes compared with co-polarized signals. However, averaging the signal during measurements has tremendously improved the image. Furthermore, in post-processing, averaging the frequency domain images and the Mueller matrix elements with respect to frequency has led to better image contrast. Some patterns in the Mueller matrix images were difficult to interpret leading to the necessity of more investigation of the Mueller matrix and its physiological interpretation of breast tumor tissues.