Conclusion
Our findings suggest that the CBM improves the efficiency of these cellulases by enabling digestion within the bulk of the film.
Results
Catalytic activity of the chimeric enzymes was enhanced up to three fold on insoluble cellulose substrates as compared to wild type. Importantly, we demonstrate that the wild type enzymes affect primarily the surface properties of an amorphous cellulose film, while the chimeras containing a CBM alter the bulk properties of the amorphous film.