Abstract
Vitamin B(12) deficiency has been shown to affect bone mass in rodents and negatively impact bone formation in humans. In this study using mouse models, we define the effect of B(12) supplementation in the wild-type mother and B(12) deficiency in a mouse genetic model (Gif(-/-) mice) during gestation on bone and muscle architecture and mechanical properties in the offspring. Analysis of bones from 4-wk-old offspring of the wild-type mother following vehicle or B(12) supplementation during gestation (from embryonic day 0.5 to 20.5) showed an increase in bone mass caused by an isolated increase in bone formation in the B(12)-supplemented group compared with vehicle controls. Analysis of the effect of B(12) deficiency in the mother in a mouse genetic model (Gif(-/-) mice) on the long bone architecture of the offspring showed a compromised cortical and trabecular bone mass, which was completely prevented by a single injection of B(12) in the B(12)-deficient Gif(-/-) mothers. Biomechanical analysis of long bones of the offspring born from B(12)-supplemented wild-type mothers showed an increase in bone strength, and conversely, offspring born from B(12)-deficient Gif(-/-) mothers revealed a compromised bone strength, which could be rescued by a single injection of B(12) in the B(12)-deficient Gif(-/-) mother. Muscle structure and function analysis however revealed no significant effect on muscle mass, structure, and grip strength of B(12) deficiency or supplementation in Gif(-/-) mice compared with littermate controls. Together, these results demonstrate the beneficial effect of maternally derived B(12) in the regulation of bone structure and function in the offspring.