Conclusion
The results of this study show that the IL-6 rs1800796 G allele, the IL-10 rs1800896 C allele, and the TNF-α rs1800629 A allele are significantly associated with an increased risk of susceptibility to GDM.
Methods
A total of 242 GDM patients and 242 healthy controls were enrolled in this study. The genotypes of the IL-6 rs1800796, IL-10 rs1800896, and TNF-α rs1800629 loci were analyzed by Sanger sequencing. Interactions among these SNPs were analyzed through multifactor dimensionality reduction.
Objective
To investigate any associations between the single nucleotide polymorphisms (SNPs) at the interleukin-6 (IL-6) rs1800796, interleukin-10 (IL-10) rs1800896, and the tumor necrosis factor-alpha (TNF-α) rs1800629 loci with gestational diabetes mellitus (GDM) susceptibility. Materials and
Results
Women with the IL-6 rs1800796 G allele had a higher GDM susceptibility risk than those with the C allele (odds ratio [OR] = 1.44, 95% confidence interval [CI]: 1.10-1.90, p = 0.010). Women with the IL-10 rs1800896 C allele had a higher risk of GDM susceptibility than those with the T allele (OR = 2.94, 95% CI: 1.87-4.63, p < 0.001). Women with the TNF-α rs1800629 A allele had a higher risk of susceptibility to GDM than those with the G allele (OR = 3.73, 95% CI: 2.25-6.18, p < 0.001). The plasma levels of IL-6 and TNF-α in GDM patients were significantly higher than those in the control group, and the levels of IL-10 were significantly lower than those in the control group (p < 0.001). Women with the IL-6 rs1800796 CG/GG genotypes had higher plasma IL-6 levels than those with the CC genotype (p < 0.05). Women with the IL-10 rs1800896 TT genotype had higher IL-10 levels than those with the TC/CC genotypes (p < 0.05), and those with the TNF-α rs1800629 GA/AA genotypes had higher TNF-α levels than those with the GG genotype (p < 0.05).