Abstract
The development of biocompatible nanotherapeutics for skin regeneration remains a major goal in regenerative medicine. Here, we report the isolation and characterization of rose petal-derived extracellular vesicle-like nanovesicles (RPDNVs) obtained by differential ultracentrifugation and size exclusion chromatography. Comprehensive physicochemical analyses confirmed their vesicular morphology, nanoscale size distribution, and antioxidant-enriched molecular cargo, including lipids, phenols, and proteins. RPDNVs demonstrated mechanical stability compatible with tissue interfacing. Functionally, they enhanced fibroblast migration and modulated extracellular matrix gene expression without inducing fibrotic responses. Their biocompatibility was confirmed by in vitro and in vivo studies on human volunteers, thus supporting their translational relevance. Notably, RPDNVs retained structural and functional stability following freeze-drying in the absence of cryoprotectants, enabling long-term storage. These results establish RPDNVs as a promising class of plant-derived nanocarriers for therapeutic skin repair.