Abstract
Identifying task-related activation in the basal ganglia (BG) is an important area of interest in normal motor systems and cognitive neuroscience. The purpose of this study was to compare changes in brain activation in the BG using results obtained from two different masking methods: a mask drawn in standardized space from a T1-weighted anatomical image and individual region of interest (ROI) masks drawn from each subject's echo-planar image (EPI) from different tasks with reference to the high resolution fast spin echo image of each subject. Two standardized masks were used: a mask developed in Talairach space (Basal Ganglia Human Area Template (BGHAT)) and a mask developed in Montreal Neurological Institute space (MNI mask). Ten subjects produced fingertip force pulses in five separate contraction tasks during fMRI scanning. ROIs were the left caudate, putamen, external and internal portions of the globus pallidus, and subthalamic nucleus. ANOVA revealed a similar average number of voxels in the EPI mask across tasks in each BG region. The percent signal change (PSC) was consistent within each region regardless of which mask was used. Linear regression analyses between PSC in BGHAT and EPI masks and MNI and EPI masks yielded r(2) values between 0.74-0.99 and 0.70-0.99 across regions, respectively. In conclusion, PSC in different BG ROIs can be compared across studies using these different masking methods. The masking method used does not affect the overall interpretation of results with respect to the effect of task. Use of a mask drawn in standardized space is a valid and time saving method of identifying PSC in the small nuclei of the BG.