Abstract
BACKGROUND: The aim was to assess the impact of non-cross-linked hyaluronic acid (HA) injections on the healing of intraoral wounds from 3 types of incisions. METHODS: A total of 36 Wister albino rats were included in this research. The rats were randomly assigned to 1 of 6 groups: the first group underwent a scalpel incision in the buccal mucosa with HA injection. The second group received a laser incision using a 976-nm diode laser with HA injection, whereas the third group was subjected to a laser incision with a 450-nm diode laser with HA injection. The fourth group underwent scalpel incision only, the fifth group received a 976-nm laser incision only, and the sixth group received a 450-nm laser incision only. Biopsies were collected at baseline, as well as on the third and seventh days, to assess wound healing using hematoxylin and eosin and Masson trichrome staining. RESULTS: Group 3 exhibited the most pronounced results on the third and seventh days after surgery, with collagen formation noted alongside well-organized granulation tissue that contributed to improved and expedited wound healing. CONCLUSIONS: In this low-volume experimental study, HA injections in wounds made with a 450-nm diode laser demonstrated encouraging outcomes, enhancing the healing process and resulting in quicker recovery.