Abstract
We present a genome assembly from an individual female Chrysops viduatus (Square-spot Deerfly; Arthropoda; Insecta; Diptera; Tabanidae). The genome sequence has a total length of 312.35 megabases. Most of the assembly (87.6%) is scaffolded into 5 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 16.08 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.