Abstract
We present a genome assembly from a tetraploid specimen of the confused eyebright, Euphrasia confusa (Streptophyta; Magnoliopsida; Lamiales; Orobanchaceae). The genome sequence has a total length of 976.50 megabases. Most of the assembly is scaffolded into 22 chromosomal pseudomolecules, supporting the specimen being an allotetraploid (2 n = 4 x = 44). There are two mitochondrial genome scaffolds with lengths of 329.69 and 112.33 kilobases, and the plastid genome is 144.97 kilobases long.