Comparative analysis of test tube and volumetric drinking monitor methods in voluntary ethanol consumption in female mice for prenatal alcohol exposure

对雌性小鼠自愿摄入乙醇的试管法和容量饮酒监测法进行比较分析,以研究产前酒精暴露对小鼠的影响。

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Abstract

OBJECTIVE: We directly compared a low-tech test tube (TT) method and a high-tech volumetric drinking monitor (VDM) to assess their impact on female mice alcohol drinking. METHOD: We adapted a voluntary drinking paradigm described by Brady et al. (2012) for both TT and VDM. Eight mice were assigned to each method, and we measured their drinking volume and blood alcohol concentrations (BACs), comparing values using t-tests. RESULTS: After 2 weeks of drinking alcohol BACs were not significantly different between the VDM (82 ± 21 mg/dL) vs.TT (73 ± 23 mg/dL) groups [t(14) = 0.27, p = 0.7935]. Calculated ethanol intake (g/kg) on the blood draw day was also not significantly different [t(14) = 0.43, p = 0.6732] between VDM (3.0 ± 0.41) vs.TT (3.3 ± 0.49) [t(14) = 0.43, p = 0.6732]. CONCLUSIONS: TT or VDM resulted in comparable average daily ethanol consumption and resultant BACs in female mice.

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