Abstract
Platelet rich fibrin (PRF) is known for regenerative properties and therapeutic potential in various medical and surgical conditions. In Otorhinolaryngology, PRF has been documented for use in repair of traumatic perforations. It has shown to accelerate healing, reduce inflammation, and improve tissue regeneration. PRF, with its higher concentration of growth factors, offers prolonged benefits in tissue repair and regeneration. This study introduces a novel technique for preparing PRF sheets and investigates their potential to enhance outcomes in endoscopic middle ear surgery, with a particular focus on healing and graft integration. We describe in detail a novel technique for preparing PRF sheets and propose their application in middle ear surgery. Clinical observations focused on epithelialization, graft integration, and the overall healing process of the mastoid cavities following surgery. The use of PRF sheets resulted in healthy epithelization and successful graft intake. Additionally, early epithelization of mastoid cavities was observed, demonstrating the therapeutic efficacy of PRF sheets in promoting tissue regeneration. The application of PRF sheets during middle ear surgery shows promising results, offering a novel approach for enhancing healing and improving clinical outcomes in endoscopic procedures. Further studies are needed to confirm these findings and optimize the technique. Level of Evidence: Level 4. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12070-025-05421-8.