High-Intensity Interval Training Reduces Adipocyte Size and enhances the Thermogenic Activity of White and Brown Adipose Tissue in Male Wistar Rats

高强度间歇训练可减少雄性Wistar大鼠脂肪细胞体积并增强白色和棕色脂肪组织的产热活性

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Abstract

BACKGROUND: This research aims to investigate and compare the effect of high-intensity interval training on some effective factors in these metabolic diseases and the possibility of signaling thermogenesis and browning in white and brown adipose tissue. METHODS: In this study, 16 male Wistar rats were divided into two control and exercise groups. The training group did HIIT exercises for 10 weeks and 5 days per week. next, the rats were euthanized, and blood samples were collected to assess glycemic indices. Additionally, samples of white and brown adipose tissue were obtained for the analysis of protein expression and histological examination. RESULTS: In white adipose tissue PGC1α expression significant increases in the exercise group (P = 0.003) also PRDM16 expression was significantly increased in the exercise group (P = 0.03) but There was no significant increase in UCP1 (P = 0.65) and There was no significantly increased AMPK the exercise group (P = 0.33). In brown adipose tissue, UCP1 expression was significantly increased in the exercise group compared to the control group (P = 0.001) but There was no significant increase in PRDM16 in the exercise group compared to the control group (P = 0.16) This research demonstrated that physical activity can enhance metabolic health, exhibiting distinct and substantial effects on both white and brown adipose tissue. However no significant effect on body weight, food consumption, and glycemic indices was observed. CONCLUSIONS: In conclusion, we show exercise can affect thermogenesis in different ways on white and brown adipose tissue.

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