Abstract
Neurons exhibit complex branched axonal morphologies in both vertebrate and invertebrate systems, and show heterogeneity in the distribution of synaptic cargo across multiple synapses. It is possible that differences in transport across multiple branches contribute to the heterogeneity in cargo distribution across multiple synapses. However, the regulation of transport at axonal branch points is not well understood. We demonstrate that branch-specific transport of synaptic vesicles is dependent on the existence of a connection between the branch and synapses. The loss of this connection causes an immediate decrease in branch-specific transport of synaptic vesicles in the PLM neuron of C. elegans .