Abstract
Developing myofibers require chemical and electrical stimulation to induce functional muscle tissue. Tissue engineering protocols utilize either or both of these to initiate differentiation ex vivo. Current methodologies typically deliver multi-volt electrical signals, which may be hazardous to developing tissues. In attempts to mimic in vivo muscle development, we stimulated cultured muscle precursor cells with a low-voltage (1 mV) digitized synaptic signal derived from cultured cortical neurons. This synaptic signal induced larger and more adherent myofibers, along with markers of myoblast differentiation, compared to those induced following stimulation with a conventional (28 V) square signal. These findings suggest that stimulation with a digitized synaptic signal may be useful in tissue engineering and physical therapy.