Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub

非豆科固氮灌木尼泊尔马桑的单倍型解析基因组组装

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作者:Shi-Wei Zhao #, Jing-Fang Guo #, Lei Kong #, Shuai Nie, Xue-Mei Yan, Tian-Le Shi, Xue-Chan Tian, Hai-Yao Ma, Yu-Tao Bao, Zhi-Chao Li, Zhao-Yang Chen, Ren-Gang Zhang, Yong-Peng Ma, Yousry A El-Kassaby, Ilga Porth, Wei Zhao, Jian-Feng Mao

Abstract

Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis.

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