Abstract
OBJECTIVE: The aim of this work was to elucidate the association of rs8108419 polymorphism in breast cancer patients in the Indian population. METHODS: This was a pilot study involving sixty-four patients with confirmed breast cancer recruited from Mahatma Gandhi Cancer Hospital and Research Institute, Visakhapatnam and twenty-six healthy individuals were the volunteer blood donors from GITAM (Deemed to be University), Visakhapatnam, with no records of breast cancer history or familial lineage of breast cancer. Blood collected from the above individuals was used to extract genomic DNA, and the PCR-RFLP was performed to analyse all the samples. RESULT: There was no notable correlation between the genotypes of rs8108419 polymorphism and clinical characteristics like patient age, tumor stage and grade, tumor type, tumor size and distant metastasis. No correlation was observed between different subtypes of breast cancer and rs8108419 genotypes. The GG and GA genotypic frequencies were 90.6 and 9.3% in tumor cases and 92.3 and 8.3% in healthy controls, respectively. The LMTK3 genotype GA was linked with a greater risk for the development of breast cancer (Odds ratio: 1.24; 95% confidence interval: 0.63-14.6; P>0.05) than the GG genotype (Odds ratio: 0.80; 95% confidence interval: 0.15-4.27; P>0.05). CONCLUSION: Overall, rs8108419 polymorphism was not associated with any clinicopathological factors in the above patients. However, the LMTK3 GA genotype was correlated with an increased incidence of breast cancer development.