Abstract
The Brown Planthopper, Nilaparvata lugens (Stål.) (Hemiptera: Delphinidae), is one of the most destructive pests of rice. Its reproductive and developmental traits are influenced by various environmental and biological factors including endosymbiotic microorganisms. Arsenophonus, a widespread endosymbiotic bacterium of insects, can affect host fitness and metabolic processes. This study investigates the role of Arsenophonus in modulating the developmental and reproductive traits of N. lugens fed on transgenic cry30Fa1 rice (KF30-14) and its parent variety Minghui 86 (MH86). Life table analysis revealed that Arsenophonus infection (Ars(+)) increased the development time and reduced the reproductive capacity of N. lugens, especially those feeding on KF30-14. The first-instar nymphs in MH86 Ars(+) (infected) exhibited slower development compared to MH86 Ars(-) (uninfected). Similarly, the third and fourth-instar nymphs in KF30-14 Ars(+) exhibited prolonged development time compared to KF30-14 Ars(-). In addition, KF30-14 Ars(+) females had significantly reduced reproductive capacity, smaller ovarian tubules and lower relative expression levels of reproduction-related genes including Trehalose transporter (Tret), Vitellogenin (Vg) and Cytochrome P450 hydroxylase (cyp314a1), while Juvenile hormone acid methyltransferase (JHAMT) expression was upregulated. RNA sequencing and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed significant enrichment of genes involved in lipid, amino acid, and vitamin metabolisms, with Long-chain acyl-CoA synthetase implicated as a key regulator of lipid metabolism and reproductive fitness. These results highlight the complex interactions between endosymbionts, host plants and pest biology, offering a solid foundation for sustainable approaches to control N. lugens in rice production systems.