Abstract
To comprehend the nutritional components and organic acids content of peppers for the purpose of advancing pepper breeding, it was imperative to analyze and compare the levels of nutritional components (capsanthin, capsaicin, dihydrocapsaicin, reducing sugar, total amino acids, crude fat, crude fiber and protein) as well as organic acids content. In this study, 18 varieties of peppers (Capsicum annuum L.) were assessed for their nutrient compositions and organic acids. variance analysis, principal component analysis (PCA), and hierarchical cluster analysis(HCA) were employed for data analysis. The levels of nutrient compositions, including capsanthin, capsaicin, dihydrocapsaicin, reducing sugar, total amino acid, crude fat, crude fiber and protein, varied within the ranges of 11.63–71.75 ug g(− 1) DW, 0.15–6.86 mg g(− 1) DW, 0.05–4.74 mg g(− 1) DW, 0.73–2.54%, 9.53–34.48%, 7.23–19.50%, 19.77–32.64% and 9.96–5.30%, respectively. Concerning 64 organic acids, 11 organic acids(3-D-hydroxybutyric acid, 3-hydroxyphenyl-hydracrylic acid, maslinic acid, 2-indolecarboxylic acid, 3,4-dihydroxyphenylacetic acid, carnosic acid, ethylmalonic acid, maleic acid, methylmalonic acid, oleanic acid and sebacic acid) were not detected. L-malic acid, cis-lactic acid and succinic acid were the main organic acids in 18 pepper varieties, ranking first, second and third respectively. Their concentrations accounted for 13.7% − 66.8%, 37.3% − 54.1%, and 3.1% − 18.3% of the total organic acid content respectively. PCA and HCA revealed that all pepper varieties formed three category, respectively. These nutritional profiles and organic acid compositions could be instrumental in pepper breeding programs aimed at developing novel cultivars, thereby expanding options for both producers and consumers. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1038/s41598-026-37078-w.