Abstract
In this study, a novel strain exhibiting heterotrophic nitrification was screened; subsequently, the strain was identified as Klebsiella pneumoniae LCU1 using 16S rRNA gene sequencing. The aim of the study was to investigate the effects of external factors on the NH(4)(+)-N removal efficiency of strain LCU1 in order to elucidate the optimal conditions for NH(4)(+)-N removal by the strain and improve the removal efficiency. The findings indicated that the NH(4)(+)-N removal efficiency of the strain exceeded 80% under optimal conditions (sodium succinate carbon source, C/N ratio of 10, initial pH of 8.0, temperature of 30 °C, and speed of 180 rpm). The genome analysis of strain LCU1 showed that key genes involved in nitrogen metabolism, including narGHI, nirB, nxrAB, and nasAB, were successfully annotated; hao and amo were absent, but the nitrogen properties analysis determined that the strain had a heterotrophic nitrification ability. After 120 h, the NH(4)(+)-N removal efficiency of strain LCU1 was 34.5% at a high NH(4)(+)-N concentration of 2000 mg/L. More importantly, the NH(4)(+)-N removal efficiency of this strain was above 34.13% at higher Cu(2+), Mn(2+), and Zn(2+) ion concentrations. Furthermore, strain LCU1 had the highest NH(4)(+)-N removal efficiency of 34.51% for unsterilised (LCU1-OC) aquaculture wastewater. This suggests that with intensive colonisation treatment, the strain has promising application potential in real wastewater treatment.