High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic stem cells

To develop an efficient freezing method suitable for large-scale cryopreservation of human embryonic stem cells (hESCs). Design: Experimental study. Setting: Research institute. Patient(s): None. Intervention(s): Two genetically modified hESC lines, H9-EF1-GFP and CHA-hES3-EF1-GFP, were cryopreserved in cryovials using a combination of two equilibration