Identifying metabolic parameters as key indicators of hyperuricemia and ischemic stroke comorbidity via interpretable Clinlabomics models

通过可解释的临床组学模型,识别代谢参数作为高尿酸血症和缺血性卒中合并症的关键指标。

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Abstract

OBJECTIVE: Ischemic stroke (IS) with hyperuricemia (HUA) correlates with poor outcomes, yet the shared pathophysiological traits remain unclear. This study examined metabolic parameters in HUA-IS comorbidity and developed an optimal interpretable Clinlabomics model for risk assessment. METHODS: A total of 2,164 IS patients and 2,459 healthy controls (HCs) were retrospectively enrolled. Participants were divided into four groups: HUA-IS (comorbidity, n=1,082), non-HUA IS (n=1,082), HUA HCs (n=1,314), non-HUA HCs (n=1,145); the latter three were defined as the non-comorbidity group. After 1:1 propensity score matching (PSM), 1,031 cases were matched in each group. Ten metabolic parameters were analyzed: serum uric acid at admission (SUA_admission), SUA on the third day of hospitalization (SUA_3d), triglyceride-glucose index (TyG), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), atherogenic index of plasma (AIP), atherogenic coefficient (AC), lipoprotein combine index (LCI), Castelli's risk index I (CRI-I), and Castelli's risk index II (CRI-II). Univariate/multivariate logistic regression, quartile-based logistic regression, and restricted cubic spline (RCS) analysis were used to explore parameters - comorbidity associations. Post-PSM data were split 7:3 into training/testing sets, least absolute shrinkage and selection operator (LASSO) regression selected features, and 11 machine learning algorithms developed Clinlabomics models. Additionally, the optimal model was validated in the testing set and an independent validation set. RESULTS: After PSM, multivariate logistic regression identified AIP as the strongest risk factor (OR = 2.74, 95%CI: 1.80-4.19). The Q4 of TyG, TG, AIP, and LCI elevated comorbidity risk (P < 0.05). Besides, RCS showed nonlinear association of LCI with comorbidity (P < 0.05). The Recursive Partitioning and Regression Trees (rpart)-based Clinlabomics model exhibited favorable performance with F1-score, accuracy (ACC), and area under the curve (AUC) of 0.960, 0.960, and 0.986. At optimal hyperparameter (cp=0.0017), the model achieved AUCs of 0.987 (95%CI: 0.982-0.993), 0.955 (95%CI: 0.939-0.972), and 0.957 (95%CI: 0.915-0.999) in the training, testing, and validation datasets, respectively, correctly identifying 87.7% non-comorbidity and 98.0% comorbidity patients in validation. SHapley Additive exPlanations (SHAP) analysis identified UA_admission, UA_3d, TyG, TG, AIP and LCI as key metabolic indicators. CONCLUSION: TyG, TG, AIP, and LCI were critical metabolic parameters for HUA-IS comorbidity, which warrant heightened attention in future comorbidity research.

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