Abstract
Background Lumbar puncture (LP) is a technically demanding procedure fundamental for diagnosis and treatment in multiple specialties. Undergraduate medical training often provides limited opportunities for deliberate practice. Video-assisted instruction and simulation-based mastery learning (SBML) have emerged as promising educational strategies. However, direct comparisons of these modalities for LP training remain scarce. Methods This randomized study included 30 final-year MBBS students assigned equally to video-assisted instruction or SBML groups. Both groups received identical learning objectives and practiced LP on simulators. Knowledge was assessed before and after training using a validated questionnaire, while procedural competence was evaluated immediately and at two weeks using the objective structured assessment of technical skills (OSATS). Data were analyzed using mixed-design analysis of variance (ANOVA) and paired t-tests. Results The video-assisted group demonstrated significantly higher immediate OSATS scores (mean ± SD: 12.73 ± 2.12) compared to the SBML group (10.47 ± 1.81; p = .004, Cohen's d = 1.05). At the two-week follow-up, SBML scores improved (11.93 ± 1.28), whereas video-assisted scores declined (11.53 ± 1.64), resulting in a significant group × time interaction (F(1,28) = 8.82, p = 0.004). Both groups achieved significant knowledge gains (mean difference = 1.17; p = 0.017). The retention index favored SBML (114%) compared with video-assisted instruction (91%). Conclusion Video-assisted instruction accelerates early acquisition of LP procedural skills, while SBML promotes superior long-term retention. Incorporating both strategies into undergraduate curricula may optimize procedural performance and durability. Such integration has the potential to improve competency development and, ultimately, patient care.