Experimentally paired high- and low-resolution confocal fluorescence microscopy dataset for deep-learning super-resolution imaging of tooth dentin porosity

实验配对的高分辨率和低分辨率共聚焦荧光显微镜数据集,用于牙齿牙本质孔隙率的深度学习超分辨率成像

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Abstract

The proposed dataset provides experimentally acquired high- and low-resolution confocal laser scanning microscopy images of dentin porosity, split into classified image patches that can be used for paired or unpaired super-resolution training. Porous dentinal tubule and branch channels represent a vast network and can be used as a model for the mechanosensory system of teeth. The large quantity and small size of tubules and branches present an imaging challenge, requiring high resolution images at a large field of view in order to image statistically relevant regions of the tooth and fully model the porosity network. Imaging more quickly increases the potential of the field of view that can be acquired, however in return decreases resolution. Deep-learning based super-resolution could be used on low-resolution images to restore high resolution information from the image. A fluorescently stained tooth slice was first imaged with 100×100 nm(2) pixel size to acquire data at the Nyquist resolution based on the theoretical point spread function of the microscope (216 nm), and low-resolutions images of the same field of view were acquired at 200×200 nm(2), 400×400 nm(2), and 800×800 nm(2) pixel sizes without changing the microscope objective to ensure image region matching. A total of six regions were imaged at 5.1 µm in depth for a total of 18 slices per region. Acquired images were registered to ensure data were paired, and images were further patched and classified as one of 3 classes based on the type and scale of porosity present in each patch. This provides a unique database of classified image patches that can be used for paired or unpaired super-resolution training, providing real examples in all resolutions.

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