Abstract
BACKGROUND: Gentamicin, an effective antibiotic, can lead to kidney damage through increased oxidative stress and inflammation. As a monoamine oxidase inhibitor, selegiline has antioxidant and anti-inflammatory properties that may reduce the negative effects of gentamicin. METHOD: This study was conducted on human kidney tubular cells (HEK) and divided into five groups, control, group of cells exposed to gentamicin, group of cells exposed to gentamicin+ 20 μmol selegiline treatment, group of cells exposed to gentamicin + treatment of cells with selegiline 30 μmol, and group of cells exposed to gentamicin + treatment of cells with selegiline 40 μmol were placed, and in all groups, the amount of gentamicin was 3 μmol. RESULTS: This research showed that selegiline increases cell viability, total antioxidant capacity, and interleukin 10 as well as decreases the amount of reactive oxygen species, malondialdehyde, and interleukin 6 against the effects of gentamicin-induced nephrotoxicity. CONCLUSIONS: These findings show that selegiline can have protective effects on kidney damage caused by gentamicin and by reducing oxidative stress and inflammation, it can be proposed as a promising treatment option to reduce the side effects of gentamicin.