High-Mortality Outbreak of Staphylococcus aureus Mastitis Associated with Poor Milking Practices in a Goat Dairy

山羊奶场不良挤奶操作导致金黄色葡萄球菌乳腺炎高死亡率暴发

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Abstract

An outbreak of mastitis caused by Staphylococcus aureus occurred in a commercial dairy goat herd during kidding season, resulting in fatal gangrenous mastitis in approximately 30% of the herd. S. aureus was recovered from milk, mammary tissue, and other organs in does subjected to necropsy. The S. aureus milk culture-positive rate among does in the hospital pen was 58.3%, while whole-herd milk cultures of clinically normal mature does identified S. aureus in 15.0% with an additional 15.0% positive for coagulase-negative Staphylococcus (CNS), yielding a total culture-positive rate of 30.0%. The prevalence of CNS in subclinical animals was consistent with previous reports from U.S. dairy goats; in contrast, S. aureus isolation rates substantially exceeded previously reported prevalences. Poor milking hygiene and milking machine dysfunction were identified as major factors contributing to the spread of the S. aureus from goat to goat. California Mastitis Test (CMT) scores were significantly higher in culture-positive does compared with culture-negative animals (p < 0.05), demonstrating the value of CMT as a practical on-farm tool for early treatment decision making. Interventions focused on addressing milking hygiene and milking machine maintenance, as well as segregation and vaccination of replacement females. S. aureus dropped to undetectable in the next two kidding seasons, whereas the CNS culture rates remained unchanged, suggesting other factors may be contributing to CNS infection. This case highlights the role of subclinical intramammary infection and milking practice factors in transmission and control of contagious mastitis pathogens like S. aureus.

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