Resistance Exercise Associated with Camu-Camu (Myrciaria dubia) and Creatine Supplementation Modulates Antioxidant Response and Cardiac Parameters in Wistar Rats

抗阻运动联合卡姆果(Myrciaria dubia)和肌酸补充剂可调节Wistar大鼠的抗氧化反应和心脏参数

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Abstract

BACKGROUND: Resistance exercise (RE) is recognized for promoting the development of muscle strength and mass, as well as contributing positively to cardiovascular health. The combination of this type of exercise with the intake of foods rich in bioactive compounds, such as camu-camu (Myrciaria dubia), and creatine supplementation may be an interesting strategy to enhance the cardiovascular system. OBJECTIVE: This study aimed to evaluate the effects of RE and supplementation with camu-camu and creatine on oxidative balance, mineral content, ATPase enzyme activity, and histological changes in the heart of Wistar rats. METHODS: Forty-eight adult rats were divided into eight groups, with or without RE. The groups received a control diet (AIN-93M), camu-camu (200 mg/kg/day), creatine (300 mg/kg for 7 days and 50 mg/kg/day thereafter), or a combination of both. The RE protocol was performed on a vertical ladder three times a week for eight weeks. At the end, the animals were anesthetized and euthanized for tissue collection. RESULTS: The trained control group that received a standard diet (AIN-T) showed greater activity of superoxide dismutase and catalase. The trained group receiving creatine and camu-camu supplementation (CC + Cr-T) showed higher total antioxidant capacity (FRAP), increased Mg(2+)-ATPase activity, higher nitric oxide levels, and a greater diameter of cardiac muscle fibers. No pathological changes were observed in heart histology in any group, indicating preservation of tissue integrity. CONCLUSIONS: RE associated with camu-camu and creatine supplementation may be an effective strategy for modulating antioxidant and functional aspects of the heart.

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