Abstract
This work implies the preparation, structural and fluorescent characterization, and application of the 1,8-naphthalimide-based fluorescent probe for H(2)S detection in human blood serum. The mechanism of fluorescent detection of H(2)S is based on the reduction of the azide group, as the chemical reactive site, in the fluorescent probe structure, to the amino group. Compound 2 possesses another important structural motif, ethanolamine moiety, which modulates physicochemical properties. The synthetic pathway for the preparation of compound 2 is consisted of two reaction steps, and the target compound was prepared with a yield of 56%. The prepared compound 2 was characterized using (1)H and (13)C NMR spectroscopy and elemental analysis. During the fluorescence spectra measurements, several influences on fluorescence intensity were investigated, including pH, time dependence, selective response, and influence of H(2)S concentration. The detection limit was calculated, which was 0.16 μmol L(-1). For examination of the application of compound 2 for H(2)S detection in real samples, compound 2 was successfully applied for H(2)S detection in human blood serum. The concentration of H(2)S in the human serum sample was 16.2 μmol L(-1).The accuracy of H(2)S determination in human blood serum samples is confirmed by the standard addition method and UV-Vis spectrophotometry method using methylene blue.