Abstract
Syngas, an industrial byproduct composed of H(2), CO, and CO(2), represents an energy-rich substrate for sustainable bioprocesses. However, the toxicity of CO limits its biological utilization to a small number of microorganisms, primarily cultured under anaerobic conditions. To expand the applicability of syngas in aerobic systems, this study investigates the use of Hydrogenophaga pseudoflava as a CO-oxidizing strain capable of converting CO into CO(2). This strain has been reported to be a suitable host strain for the engineered biosynthesis of value-added compounds such as the C(15) sesquiterpene. The amount of product synthesized depends directly on the amount of CO converted to CO(2) and subsequently assimilated as a carbon source. Under previously reported culture conditions, only low CO(2) production rates were achieved. To enhance CO oxidation and CO(2) generation as a performance indicator, both the gas feed composition and the culture medium were optimized, leading to a 75% increase in specific growth rate (0.072 h(-1)) and a 380% increase in biomass concentration (11 g L(-1)). These improvements resulted in a twofold increase in volumetric CO(2) production rate. Altogether, our findings establish H. pseudoflava as a promising platform organism for sustainable syngas bioprocesses and provide a base for further metabolic and process engineering toward bioplastic or biofuel production being part of a circular economy.