Abstract
The rapid spread of the SARS-CoV-2 in the COVID-19 pandemic had raised questions on the route of transmission of this disease. Initial understanding was that transmission originated from respiratory droplets from an infected host to a susceptible host. However, indirect contact transmission of viable virus by fomites and through aerosols has also been suggested. Herein, we report the involvement of fine indoor air particulates with a diameter of ≤ 2.5 µm (PM(2.5)) as the virus's transport agent. PM(2.5) was collected over four weeks during 48-h measurement intervals in four separate hospital wards containing different infected clusters in a teaching hospital in Kuala Lumpur, Malaysia. Our results indicated the highest SARS-CoV-2 RNA on PM(2.5) in the ward with number of occupants. We suggest a link between the virus-laden PM(2.5) and the ward's design. Patients' symptoms and numbers influence the number of airborne SARS-CoV-2 RNA with PM(2.5) in an enclosed environment.