Abstract
The growth response of Chlorella vulgaris was studied under varying concentrations of carbon dioxide (ranging from 0.036 to 20%) and temperature (30, 40 and 50 degrees C). The highest chlorophyll concentration (11 microg mL(-1)) and biomass (210 microg mL(-1)), which were 60 and 20 times more than that of C. vulgaris at ambient CO(2) (0.036%), were recorded at 6% CO(2) level. At 16% CO(2) level, the concentrations of chlorophyll and biomass values were comparable to those at ambient CO(2) but further increases in the CO(2) level decreased both of them. Results showed that the optimum temperature for biomass production was 30 degrees C under elevated CO(2) (6%). Although increases in temperature above 30 degrees C resulted in concomitant decrease in growth response, their adverse effects were significantly subdued at elevated CO(2). There were also differential responses of the alga, assessed in terms of NaH(14)CO(3) uptake and carbonic anhydrase activity, to increases in temperature at elevated CO(2). The results indicated that Chlorella vulgaris grew better at elevated CO(2) level at 30 degrees C, albeit with lesser efficiencies at higher temperatures.