Abstract
Heavy metal toxicity risk assessments in sediments often rely on pseudototal concentrations, despite the higher theoretical predictive potential of bioavailable fractions. This study introduces the Bioavailable Fraction Toxicity Factor (BTf) and the Bioavailable Fraction Toxicity Index (BTI) to evaluate metal toxicity risks using a bioavailable fraction calculated as the sum of the first two steps of the Tessier sequential extraction procedure. Investigating heavy metal pollution (Cd, Cr, Cu, Mn, Ni, Pb, Zn) in the Vetas River catchment, a critical freshwater source in the Santurbán Páramo within the Tropical Andes biodiversity hotspot, the study identified artisanal and small-scale mining as the primary driver of contamination. Water and sediment of mining areas, particularly La Baja Creek and El Volcán Village, exhibited the highest concentrations of metals, with some sediment levels being categorized as strongly contaminated by the Geoaccumulation Index and Pollution Load Index and exceeding the Probable Effect Concentration threshold. Bioavailable fraction of metals in sediments were measured. Bioavailable fractions were higher in mining-affected areas, suggesting greater potential for metal release under acidic conditions. The BTf and BTI provided a more nuanced understanding of metal toxicity risks compared to pseudototal concentrations, with higher BTI values in mining-influenced sites. These findings underscore the need for mitigation measures to address heavy metal pollution and highlight the ecological importance of the Santurbán Páramo. Further research into bioremediation potential using local flora is recommended to support sustainable management practices.