miR-196a overexpression activates the MEK/ERK signal and represses the progesterone receptor and decidualization in eutopic endometrium from women with endometriosis

miR-196a 过表达可激活 MEK/ERK 信号并抑制子宫内膜异位症女性正常子宫内膜中的孕酮受体和蜕膜化

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作者:Min Zhou, Jing Fu, Li Xiao, Shiyuan Yang, Yong Song, Xianghui Zhang, Xue Feng, Huaqin Sun, Wenming Xu, Wei Huang

Methods

Total RNA was extracted from eutopic endometrium samples in three infertile women with mild/minimal endometriosis and three disease-free control subjects. The miRNA and mRNA expression levels were analysed by microarray analysis. The miR-196a expression was validated by qRT-PCR [endometriosis (n = 22) and control (n = 20)], while functional analysis utilised in vitro transfection of endometrial stromal cells (ESCs), induction of decidualization of ESCs, and luciferase reporter assays in 293 T cell lines. Main

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