Abstract
A novel label-free fluorescence assay for detection of Hg(2+) was developed based on the Hg(2+)-binding single-stranded DNA (ssDNA) and SYBR Green I (SG I). Differences from other assays, the designed rich-thymine (T) ssDNA probe without fluorescent labelling can be rapidly formed a T-Hg(2+)-T complex and folded into a stable hairpin structure in the presence of Hg(2+) in environmental drinking water samples by facilitating fluorescence increase through intercalating with SG I in one-step. In the assay, the fluorescence signal can be directly obtained without additional incubation within 1 min. The dynamic quantitative working ranges was 5-1000 nM, the determination coefficients were satisfied by optimization of the reaction conditions. The lowest detection limit of Hg(2+) was 3 nM which is well below the standard of U.S. Environmental Protection Agency. This method was highly specific for detecting of Hg(2+) without being affected by other possible interfering ions from different background compositions of water samples. The recoveries of Hg(2+) spiked in these samples were 95.05-103.51%. The proposed method is more viable, low-costing and simple for operation in field detection than the other methods with great potentials, such as emergency disposal, environmental monitoring, surveillance and supporting of ecological risk assessment and management.