Abstract
Crafting a high-performance electrochemical sensor for chlorogenic acid (CGA) holds substantial value for advancing periodontal disease (PD) management and therapeutic strategies. In this investigation, we synthesized a graphdiyne (GDY)-platinum nanoparticle (Pt NP) hybrid (Pt/GDY) via a straightforward electroless plating technique, leveraging the complementary strengths of its components: Pt NPs mitigate GDY aggregation and enhance electrocatalytic activity, while GDY provides large surface and exceptional binding affinity toward CGA. Utilizing this Pt/GDY nanocomposite as the electrode modifier, we engineered an efficient CGA electrochemical sensor. Following optimization of critical detection parameters, including solution pH, Pt/GDY amount and incubation time, the Pt/GDY-based sensor demonstrated outstanding analytical performance, enabling ultrasensitive CGA quantification with a linear range of 0.008-2 µM and a detection limit of 2 nM. Furthermore, the sensor exhibited favorable selectivity against interfering species, long-term stability, consistent reproducibility, and reliable applicability in real samples. These superior characteristics position the Pt/GDY sensor as a promising tool for quality control assessments and drug metabolism studies in PD treatment regimens.