Abstract
Understanding the seasonality and prevalence of respiratory viruses in indoor environments is essential for protecting public health in the post-pandemic era. This study investigated the presence of airborne SARS-CoV-2 in university indoor spaces where no symptomatic or confirmed positive individuals were supposed to be present. A total of 127 high-efficiency particulate air (HEPA) filter samples were analyzed from air purifiers installed in classrooms, conference rooms, and a community room within a university building across different seasons from Fall 2022 to Summer 2023. Viral RNA was extracted and quantified using RT-qPCR for each sample. SARS-CoV-2 RNA was detected in 21% of the samples, with the positivity rate varying significantly by room type but not by season. Among the 27 positive samples, viral RNA concentrations were significantly higher in fall-winter compared to summer, with no significant differences across room types. Additionally, respiratory syncytial virus (RSV) and influenza A virus (IAV) were detected in far fewer samples (positive rates: 2% and 4%, respectively) and at much lower concentrations than SARS-CoV-2. These findings provide evidence of the potential for airborne SARS-CoV-2 transmission in shared indoor spaces, even in the absence of known infectious individuals. They also suggest that SARS-CoV-2 may circulate in each season, underscoring the continued need for interventions to reduce indoor viral exposure.