Abstract
Human enteropathy-associated T-cell lymphoma (EATL) is a rare primary aggressive intestinal T-cell lymphoma associated with celiac disease and is considered to be a neoplasm of intraepithelial lymphocytes (IELs) with an innate lymphoid cell (ILC)-like immunophenotype. The lack of an animal model has delayed the elucidation of its pathogenesis. In dogs, the histopathological and immunophenotypic features of intestinal large T-cell lymphoma (ILTCL) are similar to EATL; however, its cell of origin remains unclear. We herein performed detailed immunophenotyping, an RNA expression analysis of selected genes, and gene mutation analysis of 54 cases of ILTCL, including 27 with fresh frozen samples available and 21 of intestinal small T-cell lymphoma (ISTCL) in dogs. Canine ILTCL was characterised by the expression of cytotoxic granules (53/54) and frequent absence of CD4/CD8 (26/27) and T-cell receptors (14/27). The mRNA expression of CD103 (25/35) and NKp46 (15/35) was detected in ILTCL by RNA in situ hybridisation. The gene mutation analysis showed mutations in NFKBIA (ILTCL, 31/54; ISTCL, 10/21), including truncating mutations (ILTCL, 11/54; ISTCL, 3/21). Mutations in STAT3 SH2 were less frequent (ILTCL, 13/54; ISTCL, 3/21) and the hotspot JAK1 mutation of human EATL was not detected. Immunohistochemistry for p-Stat3 showed STAT3 pathway activation in ILTCL cases. These results suggest that canine ILTCL is also a neoplasm of IEL with an ILC-like immunophenotype and STAT3 pathway activation, and loss-of-function mutations in NF-κB pathway inhibitors are associated with its neoplastic changes. Therefore, canine ILTCL has potential as a valuable model for investigating the pathogenesis of EATL.