Abstract
Canopy ammonia (NH(3)) exchange is a major contributor to agricultural NH(3) emissions and is closely linked to nitrogen-use efficiency. Glutamine synthetase (GS) mediates plant NH(3) assimilation, yet the specific roles of different GS isoenzymes in regulating wheat canopy NH(3) exchange remain unclear. This study aimed to clarify the functional differences of wheat TaGS isoenzymes in modulating canopy-atmosphere NH(3) exchange dynamics using two wheat cultivars (Yumai 49-198 and Xinong 509) under two nitrogen application levels (120 and 225 kg N ha(-1)). Field experiments combined with FTIR-based NH(3) flux measurement, biochemical assays, and molecular analyses were conducted at anthesis and 16, 24, and 30 days after anthesis (DAA). Results showed that the leaf NH(3) compensation point, determined by apoplastic NH(4)(+) concentration, is a key factor influencing canopy NH(3) exchange. Leaf NH(3) sources exhibited distinct temporal specificity: photorespiration and nitrate reduction dominated at anthesis to 16 DAA, whereas nitrogenous compound degradation prevailed at 24-30 DAA. This temporal partitioning was highly coordinated with TaGS isoenzyme expression: TaGS2 was highest in early grain filling, potentially supporting assimilate NH(3) from photorespiration/nitrate reduction, while TaGS1;1 expression increased progressively, aligning with the scavenging of NH(3) from organic nitrogen degradation. These coordinated patterns suggest that the TaGS isoenzymes play differentiated roles in influencing wheat canopy NH(3) exchange. This study thus provides correlative insights that point to potential molecular targets for breeding nitrogen-efficient wheat cultivars and mitigating agricultural NH(3) emissions sustainably.