Abstract
Polypore mushrooms have been widely recognized for centuries for their use in food and medicine due to their strong capacity to produce numerous biomolecules with beneficial effects on human health. Fomes fomentarius is one such species that remains poorly explored, particularly when growing in Morocco. Herein, this study aimed to characterize the bioactive compounds of F. fomentarius and evaluate its pharmacological properties. Spectrophotometric analysis showed that F. fomentarius revealed high levels of total phenolics (75.83 mg GAE/g dme) and flavonoids (37.62 mg CE/g dme). Gas chromatography-mass spectrometry (GC-MS) analysis identified 109 volatile and non-volatile compounds, primarily sugars (24), fatty acids (23), alcohols (10), organic acids (9), and terpenoids (6). In addition, liquid chromatography-mass spectrometry (LC-MS) analysis allowed the identification of 24 phenolic compounds, with isorhamnetin (2734.00 µg/g), p-hydroxybenzoic acid (409.00 µg/g), and kaempferol (351.10 µg/g) as the most abundant. Regarding pharmacological properties, F. fomentarius extract demonstrated strong antioxidant activity, with the DPPH radical-scavenging assay showing the highest potency, followed by β-carotene bleaching inhibition and ferric ion-reducing power, with EC(50) (half maximal effective concentration) values of 114.40, 174.50, and 250.70 µg/mL, respectively. Additionally, it exhibited broad-spectrum antimicrobial activity against all seven human pathogenic microorganisms, with Epidermophyton floccosum being the most susceptible ((minimum inhibitory concentration (MIC)) = 2 mg/mL and minimal fungicidal concentration (MFC) = 4 mg/mL) and A. fumigatus the most resistant (MIC = 26.67 mg/mL and MFC ≥ 64 mg/mL). Overall, the result indicated that Moroccan F. fomentarius is a rich source of diverse bioactive compounds with potent antioxidant and antimicrobial activities, supporting its potential for various applications.