Abstract
In this study, whole protein hydrolysate (Madira, PRJ-1) of barnyard millet's seed was produced through a fermentation process using Lactobacillus paracasei and employed as an antibacterial hydrolysate. The antibacterial properties of the protein hydrolysate were investigated against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative) bacterial strains, respectively, using well diffusion method. Strikingly, a significant level of inhibition was observed, revealing zones of inhibition 11 ± 1.2 mm and 12 ± 1.5 mm against Gram-positive bacteria and Gram-negative respectively. Additionally, the fermented protein hydrolysate demonstrated significant antiprotease activity, with notable percentages of trypsin inhibition. The findings indicated statistically significant (P < 0.05) inhibition of microbial growth, with minimum inhibitory concentrations (MICs) 120 μg/200 μL for Staphylococcus aureus and against the Escherichia coli at pH 7.0. These results highlight the potential of whole protein hydrolysate (Madira, PRJ-1) of barnyard millet's seed as a promising bioactive hydrolysate with antimicrobial applications.