Abstract
Disinfectants play a crucial role in controlling the spread of infectious diseases caused by bacteria and spore-forming organisms. Bacteria and spores can persist on surfaces and in the environment for extended periods, posing a significant risk to public health. Disinfectants are designed to inactivate or kill these microorganisms by disrupting their cellular structures and functions. Effective disinfectants are essential for preventing the spread of infectious diseases in hospitals, laboratories, food processing facilities, and other settings where the risk of contamination is high. This study evaluated the effectiveness of a disinfectant called "MultiDez" on Y.pestis bacteria and Bacillus anthracis spores using microbiological and electron microscopic methods. Results showed that after exposure to a 0.5 % solution of the disinfectant, the death of all Y.pestis bacteria was achieved after 90 min, while the death of Bacillus anthracis spores was achieved after 240 min. Electron microscopy revealed that the disinfectant caused complete destruction of both bacterial cells and spores by enveloping their outer surfaces with polymer molecules, disrupting the structure and function of their membranes, and destroying their cytoplasm and nucleode. The mechanism of action of the disinfectant on bacteria and spores involved different processes, with the disinfectant causing rapid hydration of dehydrated spores and blocking the functions of spore membranes in the case of bacterial spores.