Abstract
We present a high-quality, chromosome-scale genome assembly for the redlip blenny Ophioblennius macclurei (family Blenniidae). The assembly was generated using a combination of Oxford Nanopore long-read sequencing, Illumina short-read data, and Hi-C scaffolding technology. The assembled genome is 529.6 Mb in size, with a scaffold N50 of 23.7 Mb and a GC content of 43.49%. BUSCO analysis recovered 97.06% of expected genes in the scaffolded assembly and 89.2% from the annotated proteome. Automatic genome annotation identified a total of 18,927 protein-coding genes. This genome provides a valuable resource for understanding the diversity and evolutionary history of Ophioblennius and the fish family Blenniidae.